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Analysis of Pseudomonas aeruginosa c-di-GMP High and Low Subpopulations Using Flow-assisted Cell Sorting (FACS) and Quantitative Reverse Transcriptase PCR (qRT-PCR)

Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger signaling molecule that drives the transition from planktonic to the biofilm mode of growth in many bacterial species. Pseudomonas aeruginosa has at least two surface sensing systems that produce c-di-GMP in response to surface attach...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Bio Protoc
Prif Awduron: Armbruster, Catherine R., Parsek, Matthew R.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Bio-Protocol 2021
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7953252/
https://ncbi.nlm.nih.gov/pubmed/33732780
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3891
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