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Analysis of Pseudomonas aeruginosa c-di-GMP High and Low Subpopulations Using Flow-assisted Cell Sorting (FACS) and Quantitative Reverse Transcriptase PCR (qRT-PCR)

Cyclic diguanylate monophosphate (c-di-GMP) is a second messenger signaling molecule that drives the transition from planktonic to the biofilm mode of growth in many bacterial species. Pseudomonas aeruginosa has at least two surface sensing systems that produce c-di-GMP in response to surface attach...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Armbruster, Catherine R., Parsek, Matthew R.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7953252/
https://ncbi.nlm.nih.gov/pubmed/33732780
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3891
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