Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts

This protocol describes the generation of protoplasts from protonemal tissue of the moss Physcomitrium patens (syn. Physcomitrella patens), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) mediated transformation. The protonemal tissue grown in liquid suspension...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado en:Bio Protoc
Autores principales: Batth, Rituraj, Cuciurean, Ilenuta Simina, Kodiripaka, Satish Kumar, Rothman, Sarah Skovlund, Greisen, Caroline, Simonsen, Henrik Toft
Formato: Artigo
Lenguaje:Inglês
Publicado: Bio-Protocol 2021
Materias:
Methods Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7952925/
https://ncbi.nlm.nih.gov/pubmed/33732756
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3782
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares