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Cellulase and Macerozyme-PEG-mediated Transformation of Moss Protoplasts
This protocol describes the generation of protoplasts from protonemal tissue of the moss Physcomitrium patens (syn. Physcomitrella patens), using Cellulase ONOZUKA R10 and Macerozyme R10, followed by polyethylene glycol (PEG) mediated transformation. The protonemal tissue grown in liquid suspension...
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| Publicado no: | Bio Protoc |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7952925/ https://ncbi.nlm.nih.gov/pubmed/33732756 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3782 |
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