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Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation(1). Due to its capacity to undergo efficient mitotic homologous recombination...

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Detalhes bibliográficos
Main Authors: Liu, Yen-Chun, Vidali, Luis
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3169274/
https://ncbi.nlm.nih.gov/pubmed/21540817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2560
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