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Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation(1). Due to its capacity to undergo efficient mitotic homologous recombination...

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Bibliografske podrobnosti
Main Authors: Liu, Yen-Chun, Vidali, Luis
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3169274/
https://ncbi.nlm.nih.gov/pubmed/21540817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2560
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