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Efficient Polyethylene Glycol (PEG) Mediated Transformation of the Moss Physcomitrella patens

A simple and efficient method to transform Physcomitrella pantens protoplasts is described. This method is adapted from protocols for Physocmitrella protonemal protoplast and Arabidopsis mesophyll protoplast transformation(1). Due to its capacity to undergo efficient mitotic homologous recombination...

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Bibliographic Details
Main Authors: Liu, Yen-Chun, Vidali, Luis
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2011
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3169274/
https://ncbi.nlm.nih.gov/pubmed/21540817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2560
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