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Kinetic and thermodynamic analysis defines roles for two metal ions in DNA polymerase specificity and catalysis
Magnesium ions play a critical role in catalysis by many enzymes and contribute to the fidelity of DNA polymerases through a two-metal ion mechanism. However, specificity is a kinetic phenomenon and the roles of Mg(2+) ions in each step in the catalysis have not been resolved. We first examined the...
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| Udgivet i: | J Biol Chem |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Biochemistry and Molecular Biology
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7948414/ https://ncbi.nlm.nih.gov/pubmed/33310704 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA120.016489 |
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