Novel RT-ddPCR assays for simultaneous quantification of multiple noncoding and coding regions of SARS-CoV-2 RNA

A hallmark of coronavirus transcription is the generation of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and an array of subgenomic mRNAs (sgRNAs) encompassing sequences arising from discontinuous transcription. Existing PCR-bas...

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Publicat a:J Virol Methods
Autors principals: Telwatte, Sushama, Kumar, Nitasha, Vallejo-Gracia, Albert, Kumar, G. Renuka, Lu, Chuanyi M., Ott, Melanie, Wong, Joseph K., Yukl, Steven A.
Format: Artigo
Idioma:Inglês
Publicat: Elsevier/North-Holland Biomedical Press 2021
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7923865/
https://ncbi.nlm.nih.gov/pubmed/33667568
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2021.114115
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