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Rapid, precise quantification of large DNA excisions and inversions by ddPCR

The excision of genomic sequences using paired CRISPR-Cas nucleases is a powerful tool to study gene function, create disease models and holds promise for therapeutic gene editing. However, our understanding of the factors that favor efficient excision is limited by the lack of a rapid, accurate mea...

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Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Watry, Hannah L., Feliciano, Carissa M., Gjoni, Ketrin, Takahashi, Gou, Miyaoka, Yuichiro, Conklin, Bruce R., Judge, Luke M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7483445/
https://ncbi.nlm.nih.gov/pubmed/32913194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-71742-z
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