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Rapid, precise quantification of large DNA excisions and inversions by ddPCR

The excision of genomic sequences using paired CRISPR-Cas nucleases is a powerful tool to study gene function, create disease models and holds promise for therapeutic gene editing. However, our understanding of the factors that favor efficient excision is limited by the lack of a rapid, accurate mea...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Watry, Hannah L., Feliciano, Carissa M., Gjoni, Ketrin, Takahashi, Gou, Miyaoka, Yuichiro, Conklin, Bruce R., Judge, Luke M.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7483445/
https://ncbi.nlm.nih.gov/pubmed/32913194
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-71742-z
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