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Endogenous 2μ Plasmid Editing for Pathway Engineering in Saccharomyces cerevisiae
In Saccharomyces cerevisiae, conventional 2μ-plasmid based plasmid (pC2μ, such as pRS425) have been widely adopted in pathway engineering for multi-copy overexpression of key genes. However, the loss of partition and copy number control elements of yeast endogenous 2μ plasmid (pE2μ) brings the issue...
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| Gepubliceerd in: | Front Microbiol |
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| Hoofdauteurs: | , , , , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Frontiers Media S.A.
2021
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7921170/ https://ncbi.nlm.nih.gov/pubmed/33664720 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2021.631462 |
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