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Genome-scale perturbation of lncRNA expression using CRISPR interference

CRISPR-mediated interference (CRISPRi), a robust and specific system for programmably repressing transcription, provides a versatile tool for systematically characterizing the function of long non-coding RNAs (lncRNAs). When used with highly parallel, lentiviral pooled screening approaches, CRISPRi...

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Detalles Bibliográficos
Publicado en:Methods Mol Biol
Main Authors: Liu, S John, Horlbeck, Max A, Weissman, Jonathan S, Lim, Daniel A
Formato: Artigo
Idioma:Inglês
Publicado: 2021
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7917014/
https://ncbi.nlm.nih.gov/pubmed/33326085
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-1158-6_20
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