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Macromolecular crowding effects on the kinetics of opposing reactions catalyzed by alcohol dehydrogenase
In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging from 10 to 550 kDa. The results reveal that the effec...
Enregistré dans:
| Publié dans: | Biochem Biophys Rep |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Elsevier
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7905371/ https://ncbi.nlm.nih.gov/pubmed/33665382 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2021.100956 |
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