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Macromolecular crowding effects on the kinetics of opposing reactions catalyzed by alcohol dehydrogenase

In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging from 10 to 550 kDa. The results reveal that the effec...

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Detalhes bibliográficos
Publicado no:Biochem Biophys Rep
Main Authors: Wilcox, Xander E., Chung, Charmaine B., Slade, Kristin M.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7905371/
https://ncbi.nlm.nih.gov/pubmed/33665382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2021.100956
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