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Macromolecular crowding effects on the kinetics of opposing reactions catalyzed by alcohol dehydrogenase

In order to better understand how the complex, densely packed, heterogeneous milieu of a cell influences enzyme kinetics, we exposed opposing reactions catalyzed by yeast alcohol dehydrogenase (YADH) to both synthetic and protein crowders ranging from 10 to 550 kDa. The results reveal that the effec...

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Dades bibliogràfiques
Publicat a:Biochem Biophys Rep
Autors principals: Wilcox, Xander E., Chung, Charmaine B., Slade, Kristin M.
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7905371/
https://ncbi.nlm.nih.gov/pubmed/33665382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2021.100956
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