Llwytho...
Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies
Aiming at streamlining GPCR production from E. coli inclusion bodies for structural analysis, we present a generic approach to assess and optimize refolding yield through thermostability analysis. Since commonly used hydrophobic dyes cannot be applied as probes for membrane protein unfolding, we ada...
Wedi'i Gadw mewn:
| Cyhoeddwyd yn: | PLoS One |
|---|---|
| Prif Awduron: | , , , |
| Fformat: | Artigo |
| Iaith: | Inglês |
| Cyhoeddwyd: |
Public Library of Science
2021
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| Pynciau: | |
| Mynediad Ar-lein: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7904181/ https://ncbi.nlm.nih.gov/pubmed/33626080 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0247689 |
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