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Refolding and characterization of two G protein-coupled receptors purified from E. coli inclusion bodies
Aiming at streamlining GPCR production from E. coli inclusion bodies for structural analysis, we present a generic approach to assess and optimize refolding yield through thermostability analysis. Since commonly used hydrophobic dyes cannot be applied as probes for membrane protein unfolding, we ada...
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| I publikationen: | PLoS One |
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| Huvudupphovsmän: | , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
Public Library of Science
2021
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| Ämnen: | |
| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7904181/ https://ncbi.nlm.nih.gov/pubmed/33626080 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0247689 |
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