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Purification of DNA repair protein complexes from mammalian cells

Cells possess multiple DNA repair pathways to tackle a variety of DNA lesions. Often, DNA repair proteins function as large protein complexes. Here, we describe a protocol to purify DNA repair protein complexes from nuclei of mammalian cells. The method permits purification of protein complexes cont...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Liang, Chih-Chao, Cohn, Martin A.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7903465/
https://ncbi.nlm.nih.gov/pubmed/33665628
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100348
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