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Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput

[Image: see text] Compartmentalization of single genes in water-in-oil emulsion droplets is a powerful approach to create millions of reactors for enzyme library selections. When these droplets are formed at ultrahigh throughput in microfluidic devices, their perfect monodispersity allows quantitati...

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Pubblicato in:ACS Synth Biol
Autori principali: Holstein, Josephin M., Gylstorff, Christian, Hollfelder, Florian
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Chemical Society 2021
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7901014/
https://ncbi.nlm.nih.gov/pubmed/33502841
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.0c00538
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