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Microdroplet-based one-step RT-PCR for ultrahigh throughput single-cell multiplex gene expression analysis and rare cell detection

Gene expression analysis of individual cells enables characterization of heterogeneous and rare cell populations, yet widespread implementation of existing single-cell gene analysis techniques has been hindered due to limitations in scale, ease, and cost. Here, we present a novel microdroplet-based,...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Ma, Jennifer, Tran, Gary, Wan, Alwin M. D., Young, Edmond W. K., Kumacheva, Eugenia, Iscove, Norman N., Zandstra, Peter W.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7990930/
https://ncbi.nlm.nih.gov/pubmed/33762663
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-86087-4
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