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Detecting aspartate isomerization and backbone cleavage after aspartate in intact proteins by NMR spectroscopy
The monitoring of non-enzymatic post-translational modifications (PTMs) in therapeutic proteins is important to ensure drug safety and efficacy. Together with methionine and asparagine, aspartic acid (Asp) is very sensitive to spontaneous alterations. In particular, Asp residues can undergo isomeriz...
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| Publicado no: | J Biomol NMR |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer Netherlands
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7897204/ https://ncbi.nlm.nih.gov/pubmed/33475951 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-020-00356-4 |
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