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Detecting aspartate isomerization and backbone cleavage after aspartate in intact proteins by NMR spectroscopy

The monitoring of non-enzymatic post-translational modifications (PTMs) in therapeutic proteins is important to ensure drug safety and efficacy. Together with methionine and asparagine, aspartic acid (Asp) is very sensitive to spontaneous alterations. In particular, Asp residues can undergo isomeriz...

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Bibliografische gegevens
Gepubliceerd in:J Biomol NMR
Hoofdauteurs: Hinterholzer, Arthur, Stanojlovic, Vesna, Regl, Christof, Huber, Christian G., Cabrele, Chiara, Schubert, Mario
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Springer Netherlands 2021
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7897204/
https://ncbi.nlm.nih.gov/pubmed/33475951
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-020-00356-4
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