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High-throughput screening of histone deacetylases and determination of kinetic parameters using fluorogenic assays
Histone deacetylases (HDACs) are ubiquitous enzymes that cleave post-translational ε-N-acyllysine modifications. The continued identification of diverse acyl modifications at lysine residues in proteins has resulted in discovery of new insight into the biological roles of these enzymes. Here, we des...
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| Pubblicato in: | STAR Protoc |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Elsevier
2021
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7890041/ https://ncbi.nlm.nih.gov/pubmed/33659897 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100313 |
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