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High-throughput screening of histone deacetylases and determination of kinetic parameters using fluorogenic assays

Histone deacetylases (HDACs) are ubiquitous enzymes that cleave post-translational ε-N-acyllysine modifications. The continued identification of diverse acyl modifications at lysine residues in proteins has resulted in discovery of new insight into the biological roles of these enzymes. Here, we des...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Moreno-Yruela, Carlos, Olsen, Christian A.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7890041/
https://ncbi.nlm.nih.gov/pubmed/33659897
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100313
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