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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing

Transposon sequencing (Tn-seq) is a powerful method that combines transposon mutagenesis and massive parallel sequencing to identify genes and pathways that contribute to bacterial fitness under a wide range of environmental conditions. Tn-seq applications are extensive and have not only enabled exa...

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Kazi, Misha I., Schargel, Richard D., Boll, Joseph M.
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7861349/
https://ncbi.nlm.nih.gov/pubmed/32716393
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/61612
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