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Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria.

We constructed the broad-host-range plasmid pUCD800 containing the sacB gene of Bacillus subtilis for use in the positive selection and isolation of insertion sequence (IS) elements in gram-negative bacteria. Cells containing pUCD800 do not grow on medium containing 5% sucrose unless the sacB gene i...

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Bibliografiske detaljer
Main Authors: Gay, P, Le Coq, D, Steinmetz, M, Berkelman, T, Kado, C I
Format: Artigo
Sprog:Inglês
Udgivet: 1985
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC214340/
https://ncbi.nlm.nih.gov/pubmed/2997137
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