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Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
Transposon sequencing (Tn-seq) is a powerful method that combines transposon mutagenesis and massive parallel sequencing to identify genes and pathways that contribute to bacterial fitness under a wide range of environmental conditions. Tn-seq applications are extensive and have not only enabled exa...
Gorde:
| Argitaratua izan da: | J Vis Exp |
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| Egile Nagusiak: | , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
2020
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7861349/ https://ncbi.nlm.nih.gov/pubmed/32716393 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/61612 |
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