Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli

The RNA-dependent-RNA polymerase (RdRp) from SARS-CoV-2 is an important drug target because it is responsible for viral RNA genome replication. Efficient production of recombinant RdRp is important in screening antivirals to treat COVID-19. Here, we present our protocol for expression of tag-free re...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:STAR Protoc
Asıl Yazarlar: Dangerfield, Tyler L., Huang, Nathan Z., Johnson, Kenneth A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Elsevier 2021
Konular:
Protocol
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7859716/
https://ncbi.nlm.nih.gov/pubmed/33558863
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100357
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