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An Improved Procedure for the Purification of the Escherichia coli RNA Polymerase Omega Subunit

We report an improved procedure for purification of the omega subunit of Escherichia coli RNA polymerase. In contrast to the original procedure, the revised procedure (i) allows purification of omega entirely from the soluble fraction, obviating the need for denaturation/renaturation, (ii) results i...

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Detalles Bibliográficos
Main Authors: Vrentas, Catherine E., Gaal, Tamas, Burgess, Richard R., Gourse, Richard L.
Formato: Artigo
Idioma:Inglês
Publicado: 2009
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2843781/
https://ncbi.nlm.nih.gov/pubmed/19963062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.11.009
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