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An Improved Procedure for the Purification of the Escherichia coli RNA Polymerase Omega Subunit
We report an improved procedure for purification of the omega subunit of Escherichia coli RNA polymerase. In contrast to the original procedure, the revised procedure (i) allows purification of omega entirely from the soluble fraction, obviating the need for denaturation/renaturation, (ii) results i...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2843781/ https://ncbi.nlm.nih.gov/pubmed/19963062 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.11.009 |
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