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Escherichia coli DksA Binds to Free RNA Polymerase with Higher Affinity than to RNA Polymerase in an Open Complex

The transcription factor DksA binds in the secondary channel of RNA polymerase (RNAP) and alters transcriptional output without interacting with DNA. Here we present a quantitative assay for measuring DksA binding affinity and illustrate its utility by determining the relative affinities of DksA for...

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Bibliografski detalji
Glavni autori: Lennon, Christopher W., Gaal, Tamas, Ross, Wilma, Gourse, Richard L.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2009
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2737966/
https://ncbi.nlm.nih.gov/pubmed/19617357
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00621-09
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