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Quantification and characterization of the 5′ exonuclease activity of the lysosomal nuclease PLD3 by a novel cell-based assay

Phospholipase D3 (PLD3) and phospholipase D4 (PLD4), the most recently described lysosomal nucleases, are associated with Alzheimer’s disease, spinocerebellar ataxia, and systemic lupus erythematosus. They exhibit 5′ exonuclease activity on single-stranded DNA, hydrolyzing it at the acidic pH associ...

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Publicat a:J Biol Chem
Autors principals: Cappel, Cedric, Gonzalez, Adriana Carolina, Damme, Markus
Format: Artigo
Idioma:Inglês
Publicat: American Society for Biochemistry and Molecular Biology 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7857491/
https://ncbi.nlm.nih.gov/pubmed/33288674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA120.015867
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