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Quantification and characterization of the 5′ exonuclease activity of the lysosomal nuclease PLD3 by a novel cell-based assay

Phospholipase D3 (PLD3) and phospholipase D4 (PLD4), the most recently described lysosomal nucleases, are associated with Alzheimer’s disease, spinocerebellar ataxia, and systemic lupus erythematosus. They exhibit 5′ exonuclease activity on single-stranded DNA, hydrolyzing it at the acidic pH associ...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Cappel, Cedric, Gonzalez, Adriana Carolina, Damme, Markus
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7857491/
https://ncbi.nlm.nih.gov/pubmed/33288674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA120.015867
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