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A Novel Dual Lentiviral CRISPR-based Transcriptional Activation System for Gene Expression Regulation in Neurons

Robust and efficient gene expression control enables the study of a gene’s function in the central nervous system. Advances in CRISPR-based technology provide new avenues not only for gene editing, but for complex transcriptional control. Here, we describe a protocol to generate high-titer lentiviru...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Savell, Katherine E., Sultan, Faraz A., Day, Jeremy J.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7854184/
https://ncbi.nlm.nih.gov/pubmed/33654850
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3348
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