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Acceleration of catalysis in dihydrofolate reductase by transient, site-specific photothermal excitation

We have studied the role of protein dynamics in chemical catalysis in the enzyme dihydrofolate reductase (DHFR), using a pump–probe method that employs pulsed-laser photothermal heating of a gold nanoparticle (AuNP) to directly excite a local region of the protein structure and transient absorbance...

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Bibliografske podrobnosti
izdano v:Proc Natl Acad Sci U S A
Main Authors: Kozlowski, Rachel, Zhao, Jing, Dyer, R. Brian
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2021
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7848751/
https://ncbi.nlm.nih.gov/pubmed/33468677
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2014592118
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