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Acceleration of catalysis in dihydrofolate reductase by transient, site-specific photothermal excitation
We have studied the role of protein dynamics in chemical catalysis in the enzyme dihydrofolate reductase (DHFR), using a pump–probe method that employs pulsed-laser photothermal heating of a gold nanoparticle (AuNP) to directly excite a local region of the protein structure and transient absorbance...
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| Gepubliceerd in: | Proc Natl Acad Sci U S A |
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| Hoofdauteurs: | , , |
| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
National Academy of Sciences
2021
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7848751/ https://ncbi.nlm.nih.gov/pubmed/33468677 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2014592118 |
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