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Optimized protocol for gene editing in adipocytes using CRISPR-Cas9 technology
We present a detailed protocol for gene editing in adipocytes using the CRISPR-Cas technology. This protocol describes sgRNA design, preparation of lentiCRISPR-sgRNA vectors, functional validation of sgRNAs, preparation of lentiviruses, and lentiviruses transduction in adipocytes. Moreover, an optim...
Tallennettuna:
| Julkaisussa: | STAR Protoc |
|---|---|
| Päätekijät: | , |
| Aineistotyyppi: | Artigo |
| Kieli: | Inglês |
| Julkaistu: |
Elsevier
2021
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| Aiheet: | |
| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7844572/ https://ncbi.nlm.nih.gov/pubmed/33554142 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100307 |
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