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Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology

Until very recently, the prospect of introducing mutations or exogenous DNA sequences at precise locations in the genomes of plants and animals was difficult, if not impossible. This rapidly changed with the demonstration that the type II CRISPR-Cas complex, a bacterial anti-viral surveillance syste...

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Detalhes bibliográficos
Publicado no:Curr Protoc Mol Biol
Main Authors: Newman, Martin, Ausubel, Frederick M.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4963815/
https://ncbi.nlm.nih.gov/pubmed/27366890
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.14
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