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Introduction to Gene Editing and Manipulation Using CRISPR/Cas9 Technology

Until very recently, the prospect of introducing mutations or exogenous DNA sequences at precise locations in the genomes of plants and animals was difficult, if not impossible. This rapidly changed with the demonstration that the type II CRISPR-Cas complex, a bacterial anti-viral surveillance syste...

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Détails bibliographiques
Publié dans:Curr Protoc Mol Biol
Auteurs principaux: Newman, Martin, Ausubel, Frederick M.
Format: Artigo
Langue:Inglês
Publié: 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4963815/
https://ncbi.nlm.nih.gov/pubmed/27366890
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.14
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