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Direct evidence for an enzyme generated LPP intermediate in (+)-limonene synthase using a fluorinated GPP substrate analog

Linalyl diphosphate (LPP) is the postulated intermediate in the enzymatic cyclization of monoterpenes catalyzed by terpene synthases. LPP is considered an obligate intermediate due to the conformationally restrictive trans-C2-C3 double bond of the substrate, geranyl diphosphate (GPP), which preclude...

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Detalhes bibliográficos
Publicado no:ACS Chem Biol
Main Authors: Morehouse, Benjamin R., Kumar, Ramasamy P., Matos, Jason O., Yu, Qi, Bannister, Austin, Malik, Karan, Temme, J. Sebastian, Krauss, Isaac J., Oprian, Daniel D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7843008/
https://ncbi.nlm.nih.gov/pubmed/31433159
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.9b00514
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