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Expression and Purification of Adeno-associated Virus Virus-like Particles in a Baculovirus System and AAVR Ectodomain Constructs in E. coli

Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape. Presented here are protocols for the production of: (a) the outer protein shells (v...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Julkaisussa:Bio Protoc
Päätekijät: Meyer, Nancy, Davulcu, Omar, Xie, Qing, Silveria, Mark, Zane, Grant M., Large, Edward, Chapman, Michael S.
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: Bio-Protocol 2020
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842819/
https://ncbi.nlm.nih.gov/pubmed/33654738
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3513
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