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High-throughput Site-directed Scanning Mutagenesis Using a Two-fragment PCR Approach
Site-directed scanning mutagenesis is a useful tool applied in studying protein function and designing proteins with new properties, such as increased stability or enzymatic activity. Creating a systematic library of hundreds of site-directed mutants is still a demanding and expensive task. The esta...
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| Published in: | Bio Protoc |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Bio-Protocol
2020
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7842796/ https://ncbi.nlm.nih.gov/pubmed/33654716 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3484 |
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