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High-throughput mutagenesis using a two-fragment PCR approach
Site-directed scanning mutagenesis is a powerful protein engineering technique which allows studies of protein functionality at single amino acid resolution and design of stabilized proteins for structural and biophysical work. However, creating libraries of hundreds of mutants remains a challenging...
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| Publicado no: | Sci Rep |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5533798/ https://ncbi.nlm.nih.gov/pubmed/28754896 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-07010-4 |
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