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Force-dependent stimulation of RNA unwinding by SARS-CoV-2 nsp13 helicase

The superfamily 1 helicase nonstructural protein 13 (nsp13) is required for SARS-CoV-2 replication. The mechanism and regulation of nsp13 has not been explored at the single-molecule level. Specifically, force-dependent unwinding experiments have yet to be performed for any coronavirus helicase. Her...

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Vydáno v:Biophys J
Hlavní autoři: Mickolajczyk, Keith J., Shelton, Patrick M.M., Grasso, Michael, Cao, Xiaocong, Warrington, Sara E., Aher, Amol, Liu, Shixin, Kapoor, Tarun M.
Médium: Artigo
Jazyk:Inglês
Vydáno: The Biophysical Society 2021
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7837305/
https://ncbi.nlm.nih.gov/pubmed/33340543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2020.11.2276
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