Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

The December 2019 outbreak of a novel respiratory virus, SARS-CoV-2, has become an ongoing global pandemic due in part to the challenge of identifying symptomatic, asymptomatic, and pre-symptomatic carriers of the virus. CRISPR diagnostics can augment gold-standard PCR-based testing if they can be m...

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Pubblicato in:Cell
Autori principali: Fozouni, Parinaz, Son, Sungmin, Díaz de León Derby, María, Knott, Gavin J., Gray, Carley N., D’Ambrosio, Michael V., Zhao, Chunyu, Switz, Neil A., Kumar, G. Renuka, Stephens, Stephanie I., Boehm, Daniela, Tsou, Chia-Lin, Shu, Jeffrey, Bhuiya, Abdul, Armstrong, Maxim, Harris, Andrew R., Chen, Pei-Yi, Osterloh, Jeannette M., Meyer-Franke, Anke, Joehnk, Bastian, Walcott, Keith, Sil, Anita, Langelier, Charles, Pollard, Katherine S., Crawford, Emily D., Puschnik, Andreas S., Phelps, Maira, Kistler, Amy, DeRisi, Joseph L., Doudna, Jennifer A., Fletcher, Daniel A., Ott, Melanie
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier Inc. 2021
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7834310/
https://ncbi.nlm.nih.gov/pubmed/33306959
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2020.12.001
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