Accurate characterization of Escherichia coli tRNA modifications with a simple method of deep-sequencing library preparation

In conventional RNA high-throughput sequencing, modified bases prevent a large fraction of tRNA transcripts to be converted into cDNA libraries. Recent proposals aiming at resolving this issue take advantage of the interference of base modifications with RT enzymes to detect and identify them by est...

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Bibliografiske detaljer
Udgivet i:RNA Biol
Main Authors: Wang, Ji, Toffano-Nioche, Claire, Lorieux, Florence, Gautheret, Daniel, Lehmann, Jean
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis 2020
Fag:
Technical Paper
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7833731/
https://ncbi.nlm.nih.gov/pubmed/32618488
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2020.1790871
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