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Accurate characterization of Escherichia coli tRNA modifications with a simple method of deep-sequencing library preparation

In conventional RNA high-throughput sequencing, modified bases prevent a large fraction of tRNA transcripts to be converted into cDNA libraries. Recent proposals aiming at resolving this issue take advantage of the interference of base modifications with RT enzymes to detect and identify them by est...

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Detalhes bibliográficos
Publicado no:RNA Biol
Main Authors: Wang, Ji, Toffano-Nioche, Claire, Lorieux, Florence, Gautheret, Daniel, Lehmann, Jean
Formato: Artigo
Idioma:Inglês
Publicado em: Taylor & Francis 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7833731/
https://ncbi.nlm.nih.gov/pubmed/32618488
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2020.1790871
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