Measuring microenvironment-tuned nuclear stiffness of cancer cells with atomic force microscopy

Quantification of nuclear stiffness is challenging for cells encapsulated within a 3D extracellular matrix (ECM). Here, we describe an experimental setup for measuring microenvironment-dependent tuning of nuclear stiffness using an atomic force microscope (AFM). In our setup, ECM-coated polyacrylami...

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Dettagli Bibliografici
Pubblicato in:STAR Protoc
Autori principali: Barai, Amlan, Das, Alakesh, Sen, Shamik
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2021
Soggetti:
Protocol
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7829340/
https://ncbi.nlm.nih.gov/pubmed/33532741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100296
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