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Measuring microenvironment-tuned nuclear stiffness of cancer cells with atomic force microscopy
Quantification of nuclear stiffness is challenging for cells encapsulated within a 3D extracellular matrix (ECM). Here, we describe an experimental setup for measuring microenvironment-dependent tuning of nuclear stiffness using an atomic force microscope (AFM). In our setup, ECM-coated polyacrylami...
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| Izdano u: | STAR Protoc |
|---|---|
| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Elsevier
2021
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7829340/ https://ncbi.nlm.nih.gov/pubmed/33532741 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100296 |
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