Flow cytometry-based ex vivo murine NK cell cytotoxicity assay

Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium rele...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:STAR Protoc
Prif Awduron: Wong, Pamela, Wagner, Julia A., Berrien-Elliott, Melissa M., Schappe, Timothy, Fehniger, Todd A.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Elsevier 2021
Pynciau:
Protocol
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7806516/
https://ncbi.nlm.nih.gov/pubmed/33490978
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100262
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