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Flow cytometry-based ex vivo murine NK cell cytotoxicity assay

Direct killing of diseased cells is a hallmark function of NK cells. This protocol describes a flow-based assay to measure in vivo activated murine NK cells’ ability to kill target cells ex vivo. Existing published protocols for assaying ex vivo NK cell killing utilized the radioactive chromium rele...

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Bibliographic Details
Published in:STAR Protoc
Main Authors: Wong, Pamela, Wagner, Julia A., Berrien-Elliott, Melissa M., Schappe, Timothy, Fehniger, Todd A.
Format: Artigo
Language:Inglês
Published: Elsevier 2021
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7806516/
https://ncbi.nlm.nih.gov/pubmed/33490978
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100262
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