Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?

OBJECTIVES: We evaluated the performance of a nucleoprotein-based enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies to SARS-CoV-2. METHODS: The ELISA was based on serum IgG reactivity to a 46-kDa protein derived from the recombinant SARS-CoV2 nucleoprotein. Assay sensitivity...

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Vydáno v:J Virol Methods
Hlavní autoři: Tozetto-Mendoza, Tania Regina, Kanunfre, Kelly Aparecida, Vilas-Boas, Lucy Santos, Sanchez Espinoza, Evelyn Patricia, Paião, Heuder Gustavo Oliveira, Rocha, Mussya Cisotto, de Paula, Anderson Vicente, de Oliveira, Maura Salaroli, Zampelli, Daniella Bosco, Vieira, José Mauro, Buss, Lewis, Costa, Silvia Figueiredo, Sabino, Ester Cerdeira, Witkin, Steven S., Okay, Thelma Suely, Mendes-Correa, Maria Cassia
Médium: Artigo
Jazyk:Inglês
Vydáno: Published by Elsevier B.V. 2021
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7804377/
https://ncbi.nlm.nih.gov/pubmed/33453299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2021.114064
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