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Nucleoprotein-based ELISA for detection of SARS-COV-2 IgG antibodies: Could an old assay be suitable for serodiagnosis of the new coronavirus?

OBJECTIVES: We evaluated the performance of a nucleoprotein-based enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies to SARS-CoV-2. METHODS: The ELISA was based on serum IgG reactivity to a 46-kDa protein derived from the recombinant SARS-CoV2 nucleoprotein. Assay sensitivity...

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Detalhes bibliográficos
Publicado no:J Virol Methods
Main Authors: Tozetto-Mendoza, Tania Regina, Kanunfre, Kelly Aparecida, Vilas-Boas, Lucy Santos, Sanchez Espinoza, Evelyn Patricia, Paião, Heuder Gustavo Oliveira, Rocha, Mussya Cisotto, de Paula, Anderson Vicente, de Oliveira, Maura Salaroli, Zampelli, Daniella Bosco, Vieira, José Mauro, Buss, Lewis, Costa, Silvia Figueiredo, Sabino, Ester Cerdeira, Witkin, Steven S., Okay, Thelma Suely, Mendes-Correa, Maria Cassia
Formato: Artigo
Idioma:Inglês
Publicado em: Published by Elsevier B.V. 2021
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7804377/
https://ncbi.nlm.nih.gov/pubmed/33453299
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2021.114064
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