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Understanding the Thermal Denaturation of Myoglobin with IMS-MS: Evidence for Multiple Stable Structures and Trapped Pre-equilibrium States

Thermal denaturation of holomyoglobin (hMb) in solution (10 mM ammonium acetate at pH = 4.5, 6.8, and 9.0) was monitored by ion mobility spectrometry (IMS) and mass spectrometry (MS) techniques to characterize the stability and investigate structural changes involved in unfolding. We utilize two exp...

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Библиографические подробности
Опубликовано в: :J Am Soc Mass Spectrom
Главные авторы: Woodall, Daniel W., Henderson, Lucas W., Raab, Shannon A., Honma, Kenji, Clemmer, David E.
Формат: Artigo
Язык:Inglês
Опубликовано: 2020
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC7790998/
https://ncbi.nlm.nih.gov/pubmed/32539412
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jasms.0c00075
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