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Hydride Transfer Catalyzed by Glycerol Phosphate Dehydrogenase: Recruitment of an Acidic Amino Acid Side Chain to Rescue a Damaged Enzyme
[Image: see text] K120 of glycerol 3-phosphate dehydrogenase (GPDH) lies close to the carbonyl group of the bound dihydroxyacetone phosphate (DHAP) dianion. pH rate (pH 4.6–9.0) profiles are reported for k(cat) and (k(cat)/K(m))(dianion) for wild type and K120A GPDH-catalyzed reduction of DHAP by NA...
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| I publikationen: | Biochemistry |
|---|---|
| Huvudupphovsmän: | , , , |
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
American Chemical Society
2020
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7784668/ https://ncbi.nlm.nih.gov/pubmed/33305938 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.0c00801 |
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