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Enzyme Architecture: A Startling Role for Asn270 in Glycerol 3-Phosphate Dehydrogenase-Catalyzed Hydride Transfer
[Image: see text] The side chains of R269 and N270 interact with the phosphodianion of dihydroxyacetone phosphate (DHAP) bound to glycerol 3-phosphate dehydrogenase (GPDH). The R269A, N270A, and R269A/N270A mutations of GPDH result in 9.1, 5.6, and 11.5 kcal/mol destabilization, respectively, of the...
Guardat en:
| Publicat a: | Biochemistry |
|---|---|
| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American
Chemical Society
2016
|
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4812618/ https://ncbi.nlm.nih.gov/pubmed/26926520 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00116 |
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