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Enzyme Architecture: A Startling Role for Asn270 in Glycerol 3-Phosphate Dehydrogenase-Catalyzed Hydride Transfer

[Image: see text] The side chains of R269 and N270 interact with the phosphodianion of dihydroxyacetone phosphate (DHAP) bound to glycerol 3-phosphate dehydrogenase (GPDH). The R269A, N270A, and R269A/N270A mutations of GPDH result in 9.1, 5.6, and 11.5 kcal/mol destabilization, respectively, of the...

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Publicat a:Biochemistry
Autors principals: Reyes, Archie C., Amyes, Tina L., Richard, John P.
Format: Artigo
Idioma:Inglês
Publicat: American Chemical Society 2016
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4812618/
https://ncbi.nlm.nih.gov/pubmed/26926520
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00116
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