Optimization of Buffer Additives for Efficient Recovery of hGM-CSF from Inclusion Bodies Using Response Surface Methodology

Overexpression of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) by Escherichia coli leads to formation of insoluble and inactive proteins, inclusion bodies. The aim of this study was to improve recovery of biologically active hGM-CSF from inclusion bodies. The effect of types, con...

Full description

Saved in:
Bibliographic Details
Published in:Iran J Pharm Res
Main Authors: Ahmadian, Mina, Jahanian-Najafabadi, Ali, Akbari, Vajihe
Format: Artigo
Language:Inglês
Published: Shaheed Beheshti University of Medical Sciences 2020
Subjects:
Original Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7758011/
https://ncbi.nlm.nih.gov/pubmed/33680031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.22037/ijpr.2020.1101169
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items